Zolotaryova T.A.

Institute of Medical Rehabilitation, Odessa, Ukraine

Development of treatment modalities is usually based on two approaches — experimental trials and clinical trials. A survey of general biological and physiological response to application of physical agents is performed on intact animals, while the study of its influence on pathogenesis of different diseases is performed mostly on animals with experimental pathology models. In our institute the department of experimental research functions more than 50 years, and during the years of its existence a great deal of experimental studies of effects of natural and artificial physical agents have been performed. The main objective of these investigations involved biological and therapeutic effects of medicinal mud, mineral waters and physiotherapeutic agents. Several particular examples are herewith presented for consideration of clinical benefits of our experimental trials. In our experimental trials we discovered the increase in catalytic activity of hepatic monooxygenases including CYP-450IIAI under the influence of low-intensity infrared laser application over hepatic region that accompanied hypocholesterolemic effect in rats. This effect of low-intensity infrared laser irradiation has been confirmed in patients with hypercholesterolemia in clinical trials. It is well known that mud application causes an antioxidant effect, which partly provides its antiphlogistic effect, but it is of interest to know which of the factors plays a key role in induction of antioxidant activity in vivo. We established that antioxidant effect of mud in rats with immobilization-cold stress model depends on mode of application. When solely mud thermal factor was involved, the changes in activity of prooxidant-antioxidant system in rats with experimental model were similar to ones in rats with experimental model only. When both factors of mud application were involved the activity of prooxidant reactions appeared to be limited but activity of antioxidant enzymes was significantly elevated. The clinical implication of this experimental data lies in limitation of temperature regimes of mud applications when used for treatment of diseases in pathogenesis of which prooxidant reactions play a principal role. Mineral waters are widely used as an additional treatment modality for addressing metabolic and functional disorders in patients with various pathologies. In our trials it was shown that intake of mineral water containing boron in rats with experimental impairment of glucose tolerance provided normalization of glucose tolerance test. This experimental data allows two general conclusions to be drawn: firstly, it is feasible to consider mineral water intake as a preventive modality in relevant patients, and secondly, mineral waters which contain boron are good for addressing impairment of glucose tolerance.In conclusion it is worth to underline that experimental trials form a basis for new physical agent treatment modalities and advance the effectiveness of recuperative treatment.